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| Laranja,Alessandra Theodoro; Manzatto,Antonio José; Campos Bicudo,Hermione Elly Melara de. |
| Caffeine and used coffee grounds completely blocked the development of Aedes aegypti in the early stages, in treatments with the concentrations 1.0 mg/mL and 50 mg/mL, respectively. More advanced stages and even adults were obtained in lower concentrations of both substances, enabling observations to be made of mortality rate, longevity and esterase patterns. The experiments involved treatments using either eggs or 3rd instar larvae (L3), with or without the addition of fish food. Mortality rates prior to the adult stage and adult longevity were significantly different in the comparisons among treatments, in every kind of experiment, but in those using L3 larvae, their percentages were smaller. Observations of the time of larva and adult onset suggested... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aedes aegypti; Caffeine; Used coffee grounds; Mortality; Longevity; Esterases; Alternative control. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400004 |
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| Rebechi,Débora; Richardi,Vinicius S.; Vicentini,Maiara; Guiloski,Izonete C.; Assis,Helena C. Silva de; Navarro-Silva,Mário A.. |
| Low malathion concentrations influence metabolism in Chironomus sancticaroli (Diptera, Chironomidae) in acute and chronic toxicity tests. Organophosphate compounds are used in agro-systems, and in programs to control pathogen vectors. Because they are continuously applied, organophosphates often reach water sources and may have an impact on aquatic life. The effects of acute and chronic exposure to the organophosphate insecticide malathion on the midge Chironomus sancticaroli are evaluated. To that end, three biochemical biomarkers, acetylcholinesterase (AChE), alpha (EST-α) and beta (EST-β) esterase were used. Acute bioassays with five concentrations of malathion, and chronic bioassays with two concentrations of malathion were carried out. In the acute... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Acetylcholinesterase; Bioindicator; Esterases; Organophosphate; Toxicity. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262014000300012 |
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| Mateus,Rogério Pincela; Cabral,Hamilton; Bonilla-Rodriguez,Gustavo Orlando; Ceron,Carlos Roberto. |
| A method that allows the measure of molecular weight of two well-known and closely related esterases from Drosophila mojavensis and its sibling species, D. arizonae, is here described, using native polyacrylamide gel electrophoresis at several concentrations, applying Fergunson´s principles. These enzymes, namely EST-4 and EST-5, presented molecular weight values between 81 and 91 kDa. In spite of their distinct expression pattern through the insect's life cycle, they showed properties of isoenzymes codified by distinct structural genes, supporting the hypothesis of a rather recent gene duplication event that generated both in D. mojavensis and D. arizonae, as well as in other species of repleta group. The method is simple and adequate to be applied to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Esterases; Isoenzymes; Molecular weight determination; Drosophila mojavensis; Drosophila arizonae; PAGE. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500004 |
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| Benedito,M.A.C.. |
| Acetylsalicylic acid (ASA), the most used drug worldwide, is hydrolyzed to salicylic acid and acetate by esterases present in tissues of several species including humans. Sex differences in drug metabolism by rodent liver are documented in the literature. In this paper we report a difference in the activities of the esterases (ASA-esterase I and II) in the kidneys of male and female mice. In this species there is no difference between males and females in liver ASA-esterases (ASA-esterase I: males 38.5 ± 7.9 (N = 5) and females 31.6 ± 7.6 (N = 5) nmol of salicylic acid formed min-1 mg protein-1, P>0.05; ASA-esterase II: males 77.3 ± 17.4 (N = 5) and females 61.4 ± 15.1 (N = 5) nmol of salicylic acid formed min-1 mg protein-1, P>0.05).... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Acetylsalicylic acid; Esterases; Mice; Sex differences; Kidneys; Liver. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000300004 |
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